FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79871302
Sequences flagged as poor quality0
Sequence length126
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTGG13300.057.76962120
GAGTGTG2850.054.760788120
GCGTCGG62450.051.231365120
TCGTGTG31900.050.994083120
TAAGATG1650.043.655453120
TTCGGTG705.1791645E-542.87589120
AAAGAGG46200.041.446693120
GGAAAGG39050.039.966255120
TGGTCCC5700.038.9569942-43
TCCGTGG7650.038.83347338-39
AGAGCGG40300.038.279766120
GGATCCT1500.038.00565350-51
TCTCGGG9750.037.55488120
GCCGGAT3850.037.40800546-47
CGTGGTC7900.037.22496440-41
CCGTGGT8300.036.8769138-39
GTGGTCC5400.036.676340-41
GGATCAT2100.035.71959750-51
GGTGGTG4450.035.071514120
GTAGGGG25400.034.739597120
Produced by FastQC (version 0.11.5)