FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44338763
Sequences flagged as poor quality0
Sequence length126
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT638730.1440567929240606No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTC1600.063.74489120
ACGCTCT1051.0186341E-1051.531961
CTTCCGA1051.03682396E-1051.448476
TCCGATC1101.6370905E-1049.0860338
GGCCGTC1003.8598955E-947.996155120
GTCCAGT3400.047.64324120
CGCACAA909.120777E-846.662926120
CTCACGC558.190751E-443.632866120
CTAGTCC558.190751E-443.632866120
CAACTCC3850.040.516235120
CTGCCGT1551.10958354E-1038.706573120
GCAGTCA3150.038.092186120
CTCTAAC801.1411521E-437.496994120
CAAGAGC4650.037.41636120
CGCTCTT1452.415618E-937.315742
CCGATCT1503.434252E-935.9964269
CCATACC1208.7151966E-734.997196120
CGAGCAC3150.034.282967120
CACGCAA350.002679278434.282307118-119
ACTCGCT350.002679278434.282307116-117
Produced by FastQC (version 0.11.5)