FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30209871
Sequences flagged as poor quality0
Sequence length126
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGAA358.569746E-568.9151120
CCGATAC350.00739674251.686325120
CCCTCGA700.002613098134.45755120
CCGTACA551.9050107E-532.784573118-119
CACGGAC750.00366420432.16038120
AACGCCC953.0403346E-431.737217120
TCGCATG3950.029.005405120
CGACACC1305.8280213E-527.831099120
TACTACC1305.8280213E-527.831099120
ACACCGC2403.7471182E-1027.637827120
CTTGCCG557.3942257E-427.267405114-115
CAACGGA1357.535514E-526.800316120
GCGTACA900.00893126626.800316120
CTACGCT450.00907426.713352118-119
GTACGCC3200.026.381561120
CACGTAG6050.025.914354120
TACCCGC600.001214836525.043768118-119
TCGCCTG600.001228684124.995123114-115
CCACGAA1451.2248175E-424.952019120
AACTACC3800.023.802912120
Produced by FastQC (version 0.11.5)