FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28064609
Sequences flagged as poor quality0
Sequence length126
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA489880.17455436489423387No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGG7950.040.758133120
TCTCGGG12200.040.33125120
CGGTGGG14650.036.45357120
ATGTGAG1001.0032398E-536.00302120
GCATCGG851.6285451E-435.297077120
CAATGGG750.003753460732.002686120
GCAGCAG1004.2267508E-430.002518120
GTGGTCC1402.9003586E-630.002516120
CTAGGGG1203.491729E-530.002516120
CAGAGCC1453.8120907E-628.967945120
GTCATGA1055.624066E-428.573824120
GCGTCGG56900.028.051561120
TCGTGTG25800.027.444162120
GCTGATT450.0091479726.668903118-119
GTTGACG709.97812E-525.716442118-119
GGAGATT1409.977946E-525.716442120
GTAGATT10350.025.509384120
GCCGTGG1651.04054725E-525.45668120
AAAGAGG82800.025.074566120
AGTCAGC600.001226686425.002098118-119
Produced by FastQC (version 0.11.5)