FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5440045
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT141790.26064122631338527TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA29150.050.1159481
ATCGGAA29450.049.4209442
TCGGAAG30800.047.5089533
CGGAAGA32700.044.30534
TATGCCG19150.038.43958748-49
CGTATGC19900.036.99017346-47
CTCGTAT19750.036.79036744-45
TCTCGTA18550.036.2266642-43
TCGTATG20350.035.93900744-45
TGCCGTC21750.033.62584750-51
ATGCCGT22250.032.87051448-49
ATCTCGT20700.032.69341742-43
GCCGTCT22550.032.1170150-51
CCGTCTT23300.031.2890452-53
CGTCTTC23150.030.87629752-53
CGTCTGA24200.030.41726316-17
ACACGTC25950.028.73175212-13
AGAGCAC53150.027.7882338
ACGTCTG27650.026.7934914-15
AATCTCG25450.026.68492740-41
Produced by FastQC (version 0.11.5)