FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10084115
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN139880.1387132138021036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG33800.050.2113384
TGCCGGG75700.049.7795683
AACGCGC34200.049.23022525
GGCGCGT163850.049.0741216
TACGGCC35700.047.742557
GCGCGTG168850.047.70317
TCTACGG36400.047.5836645
CGCGTGC169400.047.34549318
TGGCGCG172350.046.9170415
TTGCCGG34300.046.6661382
GCGTGCC173800.046.0682819
CGGTTCA23900.044.931725
GAACGCG37750.044.69328724
GCGCGGT186150.044.2937059
GTGGCGC183100.044.18229314
ATCTCGT38450.043.91299435
CTACTCG182250.043.6689537
CGCGGTG191600.043.48582510
CGACTCT61300.043.110844
CCCGATC39100.042.898231
Produced by FastQC (version 0.11.5)