FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11121299
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5594305.0302577064064184No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT585870.5267999718378222No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCG402380.361810252561324No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG347300.31228366398565494No Hit
CCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGACGAGATCGGGCGCGTTCAGGGTGGT338310.3042000759083988No Hit
GCCAGTCAAATTTAGCAGTGGGGGGTTGTATACCAACTTTAGTGACACTA249050.22393966747949137No Hit
GCACCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT243080.2185715895238497No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG238520.21447134907531937No Hit
GCCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA230260.20704415914004293No Hit
GTTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT218770.19671263222039082No Hit
CGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG216440.1946175532192777No Hit
GACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGT211870.1905083210153778No Hit
AAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGACGAGATC210480.18925846701900562No Hit
GCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCT203600.18307213932473176No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT197940.17798280578554718No Hit
GCCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT173500.15600695566228368No Hit
GCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCG166090.1493440649334219No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163190.1467364558762425No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG160410.14423674788349813No Hit
TGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTG159390.14331958883580057No Hit
AGCCAGTCAAATTTAGCAGTGGGGGGTTGTATACCAACTTTAGTGACACT155780.1400735651473807No Hit
AGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGT155580.13989373003998903No Hit
CAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAG151340.1360812257632854No Hit
CCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGACGAGA151030.13580248134682826No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT149290.13423791591252066No Hit
ACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA145790.13109080153316624No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT143150.12871697811559604No Hit
GCTCTGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT135210.12157752435214626No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA133910.12040859615410034No Hit
GGGCCTGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT130980.11777401183081221No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG124780.11219912350167009No Hit
CCCATTCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGACGAGATCGGGCGCGTTCAGGGTGGT120100.10799098198870473No Hit
GGGCCAGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT118510.10656129288494087No Hit
AGCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCG113090.1016877614746263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT143500.072.1445770
GCACCGT40750.054.0582121
CACCGTT41100.050.7840652
ACCCACT57300.048.2130570
GATCCGT15000.046.104011
ATGGCCG168850.045.65440869
AGGACCG34500.043.21798370
ACGTACG16650.041.47564357
GTCCGTT20500.041.2855872
CCTATCG3950.039.2680552
CGTACGA18350.038.3311258
CTACGTA18300.037.5144955
AGTTCAC91600.036.5169756
ATTTCCG25250.036.189836
ATCCGTT19400.036.1526572
GTTCCGT50900.036.120591
CTACGTT16150.035.9115832
CTACACG20600.035.4670249
TACGTAC19900.034.79245456
TAGATCG19100.034.41878529
Produced by FastQC (version 0.11.5)