FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9698015
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4288774.422317350509357No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT275970.28456338745609283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT59700.043.39702270
AGGACCG49050.040.47966470
CTGGCGC28850.037.8425470
GACGTAT32900.032.75104544
CCTATCG4550.032.237352
GGACGTA33750.031.802343
ACGTATC35150.030.66831645
CGTATCA35150.030.07708546
AGGACGT35900.029.79701842
CGGATAG36200.029.61743735
ATGGCCG67900.029.5499169
GCACCGT14150.028.991811
TCGGATA37800.028.55152334
CGGACTA4650.027.242025
AGTCCGC31300.026.939317
CGACGTT5000.026.0019323
CAGTCCG31800.025.892776
CGCGGAT4950.025.5911273
GCGAGGT31750.025.22762960
GATATTA44300.024.952653
Produced by FastQC (version 0.11.5)