FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9501213
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN740210.7790689462492841No Hit
CCCGGTTTGTATTCGTACTGAAAATCAAAATCAAGTCAGCTTTTACCCTT157870.16615773164963252No Hit
GTCCCTATTAATCATTACCTCTTTGCGCAAACCAACAAAATAGCAAAAAA145400.15303309166945317No Hit
ACCCGTTAAAGCCATGGTAGGCCAATACCCTACCATCCAAAGCTGATAGGGCAGAAACTTGAATGATCCATCGCC116220.12232122361639508No Hit
TCCCGTTACCCGTTAAAGCCATGGTAGGCCAATACCCTACCATCCAAAGC102290.10765993773637114No Hit
GCTGCCTTCCTTGGATGTGGTAGCCGTCTCTCAGGCTCCCTCTCCGAAAT101800.1071442141124507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTAAT284350.049.4581370
GGCCCTA21800.047.7652971
GACCCGT9600.047.252961
AGGGTAT43250.040.92222670
GAGTCGG32400.037.07357870
CAGATGT42050.036.9077870
ATGCTAA300650.036.83810869
TCGGCCA49350.036.6063542
GACCCTA20400.035.8819271
TACCAAG76400.035.2913344
TGCCCGT15250.034.929161
GTCCTTA15650.034.9147641
AAGGGTA37950.034.77523869
GTACCAA118400.034.32688543
GGTCCCT14100.034.122061
CAAGGTC81400.033.2826647
CCAAGGT143750.032.69940646
GGCCTAA4100.032.675192
GGCCCGT11050.032.6552731
AAGGTCC82900.032.5493548
Produced by FastQC (version 0.11.5)