FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9501213
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN739500.7783216732431953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACCA39100.053.40959513
CCATACC39650.052.66733612
GCGCGGT42400.051.517219
TCTACGG22950.049.7472275
ACCATAC19400.049.31498711
TACGGCC23900.048.6341557
ACGGCCA24350.048.5802428
GCCATAC23950.048.53674311
CTACGGC24700.046.7795876
AAGTAGC35700.046.3859255
CGGCCAT25900.046.3390359
GCTACTC48700.046.178836
CGCGGTG47650.045.91235710
AACGCGC24950.045.765325
ACGCGCC24900.045.4423826
GCGCGTG49300.045.41471517
CGACCAT21300.045.0772259
GGCCATA26550.045.0746910
TCTCAGC28100.044.5375257
CGCCCGA25600.044.20239629
Produced by FastQC (version 0.11.5)