FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9682004
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4029514.161855334907939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG151250.058.874915
GTCTACG151400.058.80774
CTACGGC152950.058.0274966
TACGGCC153150.057.6492847
CCTGGTT230600.056.50000870
ACGGCCA156250.056.485068
CGGCCAT156600.056.1050539
GCCATAC160550.054.20499811
GGCCATA166000.053.108510
CATACCA169100.050.9892313
CCATACC171100.050.37315412
TGTCTAC80150.050.1503643
GGTCTAC61200.048.3864023
TACCACC191250.045.0661115
TCGACTC19900.044.625153
AGTCTAC35750.043.7039763
ATACCAC200250.043.44112814
TTTCGAC12000.043.1604161
ACCACCC202650.042.44693416
AACGCGC194950.042.1904425
Produced by FastQC (version 0.11.5)