FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10054849
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN627780.6243554726679635No Hit
CCCGGTTTGTATTCGTACTGAAAATCAAAATCAAGTCAGCTTTTACCCTT163880.16298603788082747No Hit
GTCCCTATTAATCATTACCTCTTTGCGCAAACCAACAAAATAGCAAAAAA143910.14312497383103415No Hit
ACCCGTTAAAGCCATGGTAGGCCAATACCCTACCATCCAAAGCTGATAGGGCAGAAACTTGAATGATCCATCGCC138770.13801301242813294No Hit
GCTGCCTTCCTTGGATGTGGTAGCCGTCTCTCAGGCTCCCTCTCCGAAAT130840.13012627041937677No Hit
TCCCGTTACCCGTTAAAGCCATGGTAGGCCAATACCCTACCATCCAAAGC118400.11775413037033178No Hit
TACCCGTTAAAGCCATGGTAGGCCAATACCCTACCATCCAAAGCTGATAG111110.1105038971743882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCTA23550.053.0640341
TGCTAAT331600.047.89855270
GACCCGT10700.047.6170541
GGTCCCT13600.045.310481
AGGGTAT43200.045.18031770
GGCCCGT11750.039.2601051
TGCCCGT16150.039.0088461
GACCCTA22650.037.2376631
TCGGCCA53600.037.19559542
AAGGGTA39100.036.96535569
CCCCCTG25500.036.0458831
CTGGAAC25250.035.7037285
CAGATGT37200.035.70100470
ATGCTAA350800.035.21256669
GTGCCTA9100.035.1824531
GTAGCTG32800.035.08054470
TACCAAG91500.034.611844
CCCTGGA24900.034.4092983
CTCGGAA14050.033.7968644
GAGTCGG38950.033.54480470
Produced by FastQC (version 0.11.5)