FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005333264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11433300
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5048094.415251939510028No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT573840.5019023379076907No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCG418280.3658436321971784No Hit
CCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGACGAGATCGGGCGCGTTCAGGGTGGT392090.34293685987422706No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG377020.32975606342875635No Hit
GCCAGTCAAATTTAGCAGTGGGGGGTTGTATACCAACTTTAGTGACACTA288230.25209694488905215No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG259670.2271172802253068No Hit
AAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGACGAGATC246120.21526593371992336No Hit
GCACCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT245170.21443502750736884No Hit
GCCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA230240.2013766803984851No Hit
GACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGT225160.19693351875661444No Hit
CGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG222370.1944932784060595No Hit
GTTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT219130.19165945090218922No Hit
GCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCT207250.18126875005466486No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT205040.17933579981282743No Hit
GCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCG195600.17107921597439057No Hit
CCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGACGAGA181160.15844944154356136No Hit
AGCCAGTCAAATTTAGCAGTGGGGGGTTGTATACCAACTTTAGTGACACT178860.15643777387106086No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG178310.15595672290589768No Hit
GCCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT172240.1506476695267333No Hit
CAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAG171170.14971180673996135No Hit
AGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGT170420.1490558281511025No Hit
TGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTG158370.1385164388234368No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151920.1328750229592506No Hit
ACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA150280.1314406164449459No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT150190.13136189901428283No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT148660.13002370269301078No Hit
CCCATTCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGACGAGATCGGGCGCGTTCAGGGTGGT143480.12549307723929223No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA135040.11811113151933388No Hit
GCTCTGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT133970.1171752687325619No Hit
AGCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCG129870.11358925244680015No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG129070.11288954195201736No Hit
GGGCCTGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT125920.11013443187881014No Hit
CAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAGACGAGAT124510.10890119213175548No Hit
CCCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCAG122330.1069944810334724No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)