FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11433300
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5020474.391094434677653No Hit
TTTGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG392830.3435840920819011No Hit
GTTGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG375410.328347896058006No Hit
GGTGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG310700.2717500634112636No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG284520.248852037469497No Hit
TTTGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC276720.24202986014536487No Hit
GTTGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC271930.23784034355785294No Hit
TGTGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG262990.23002107877865535No Hit
TTTGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC261910.22907646961069858No Hit
GTTGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC255040.22306770573675144No Hit
GTGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG251400.21988402298548973No Hit
GGTGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC250500.21909684867885915No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC245630.21483736104186893No Hit
GGTGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC233920.20459534867448592No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC225080.19686354770713618No Hit
TTGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG192040.1679655042726072No Hit
TGTGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC186910.1634786107248126No Hit
GTGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC185970.16265645089344283No Hit
TGTGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC182150.15931533328085504No Hit
GTGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC174740.1528342648229295No Hit
TGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG158750.1388488013084586No Hit
GTTCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT143060.12512572922953127No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT141610.12385750395773748No Hit
TTTCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT141090.12340269213612867No Hit
AGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC134390.11754261674232287No Hit
ATTGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG133860.11707905853952927No Hit
TTGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC132020.11546972440152888No Hit
AGTGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC131710.11519858658480053No Hit
GGTCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT129720.11345805672902835No Hit
AGTGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC126970.11105280190321254No Hit
TTGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC124930.10926854014151645No Hit
AGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC124390.10879623555753806No Hit
AGTGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG123440.1079653293449835No Hit
ATTGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC115360.1008982533476774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGTT605000.086.21480670
CCGAACT116100.067.11371667
GCCGAAC115450.066.8611266
CGAACTT124100.064.86462468
TGCCGGG380550.063.3269163
CCATATT123650.061.96313556
CACCATA123000.061.80976554
ACCATAT124250.061.12687355
CATATTG125400.061.06230557
CGGTTCA120900.061.055445
CTTAGGC120900.060.67527818
GCCTGGT647350.060.10368769
CCTTAGG122300.059.92321817
TCCGGTT70650.059.802863
GTGTCTA94000.059.246092
TAGGCAA124550.059.16922420
TGTCTAC238700.059.1044963
TCCTTAG124200.059.0880616
GTCTACG497600.058.8676384
TCTACGG500700.058.6947635
Produced by FastQC (version 0.11.5)