FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12944703
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN145908811.271699319791269No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293570.2267877447632441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG77700.036.74466370
TGGCCGT65200.035.36425870
CTGGCGC20150.035.33844870
CGCGGAT9250.035.2482763
CCTATCG7600.033.5038222
CGTCGTT7600.032.686373
ATGGATA26200.031.28866469
CTGTCGA9350.029.2254623
CGCGACT4450.028.6100062
TATCGAT9150.028.1673134
GCACCGT19100.027.9761261
CGCTGTT16150.027.8795553
GGCGTTT10650.027.6988954
GACGTTT10700.027.569464
GACGTAT39000.027.1332444
CGTATCA39650.026.91536946
CCTGTCG10550.026.7844682
GTCGTTT10450.026.743284
ATGGCCG72150.026.63412769
AGTCCGC32600.026.4737577
Produced by FastQC (version 0.11.5)