FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10094511
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCC37650.026.84251470
TCGTACG13950.025.1396612
CGATCCG17700.024.86695965
ACGTACG4700.023.72903657
GCACCGT18300.022.9740371
GATCCGA18250.022.80076666
AGAGTCG28450.022.4559068
CGTGATA13800.022.3976867
GCGTACG6100.022.2255942
CGTACGG6350.021.90193243
CCGTTAT6050.021.8136734
CGTACGA16100.021.78347413
AGTCGTA16800.021.70087210
GACCCGT7700.021.6597541
GTACGAG16350.021.23801214
GTCGTAC17300.020.87297811
CCGATCG24250.020.62491270
ACGACGC16400.020.57121345
CGACCGT40400.020.36863920
GTTCGAC40000.020.31332617
Produced by FastQC (version 0.11.5)