FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10094511
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT161550.050.973499
CGCGGTG171600.048.109210
GGCGCGT172600.047.8056316
TGCCGGG78700.047.411453
TGGCGCG173850.047.3051115
TGCGACA54900.045.8713324
CGATCTG56500.045.5519215
GCGCGTG182500.045.21166617
CCGAACT23750.045.09181667
CGCGTGC183150.044.97902718
TTGCCGG34900.044.6294672
GCGACAT56500.044.4484325
GCGTGCC186150.044.40497219
CTACTCG185850.044.03516837
GTGGCGC191650.043.2364414
TACTCGG190650.043.02029438
AACGCGC18950.043.01549525
CGTGCCT192550.042.94790620
TAGTCCC192700.042.5542428
CGGTTCA25300.042.494445
Produced by FastQC (version 0.11.5)