FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10574646
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCG106350.10057074250996205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGATA14700.027.00158967
CGATCCG21500.025.02471465
CGCGGCA17250.023.95732132
GCACCGT17700.023.9453471
CGTACGG6250.022.80913443
CCGATCC39300.022.67261570
GATCCGA22600.022.59397966
ACGTACG5350.022.15511557
GCGTACG6450.021.56014642
TGTACCG26500.020.97145334
TTACCGT26950.020.86936419
GTACCGC27600.020.38993535
TTCCGAC78000.020.3847458
CGACCTG78550.020.37537611
ACGCCGT8200.020.3358351
TACCGTT28050.020.29830720
AGTCCGC22000.020.0409157
TACCGCC27600.020.01433436
TTCGTGA19950.019.96405665
TCGTACG13750.019.94736712
Produced by FastQC (version 0.11.5)