FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10385925
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN122330.11778440533703063No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT109500.10543114840517334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCCG46850.030.94750870
CGTGATA18100.028.39773267
ACGTACG7800.028.18015357
CGCGGCA12650.027.71087532
TAGGTAC37450.027.14348611
TAGATCG8000.026.88782729
GCGTACG13450.026.35716442
CGTACGG14000.025.32733243
TTACCCG12650.024.6827439
CGTACGA8950.024.56876858
GGCGTAC15000.023.85999341
TTCGTGA21550.023.71459465
GTTAGGT44250.022.7366339
CGACCCG16300.022.56139623
CGCGAAG8350.022.4361722
GCGATCG10700.022.41726343
CGTGCGA10900.022.31171840
GTGGGTT63800.022.147521
TACCCGC14400.021.92444810
ACGGCTA16650.021.30870446
Produced by FastQC (version 0.11.5)