FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8812211
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN641790.7282962244095155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATACC38250.054.2444712
CATACCA38950.053.26868413
GCGCGGT37250.052.938989
GCCATAC22650.050.72366311
ACGGCCA22850.049.8257188
TCTACGG22900.049.1117555
CGCGGTG40500.048.5211510
CGACCAT20850.047.207379
ACCATAC20500.047.17688411
CGCCCGA23600.046.93718729
CTACGGC24150.046.856386
AACGCGC23900.046.49124525
CGGCCAT25400.046.447119
TACGGCC24250.046.3830037
AACGACC21450.046.042827
TCTCGTC24500.045.9808536
CCCGATC24300.045.63727631
GCGCGTG43700.045.59307517
ACGCGCC24550.045.5378826
GCTACTC44550.045.1606836
Produced by FastQC (version 0.11.5)