FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11300077
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1764641.5616176774724633No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT858460.7596939383687386No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA197480.17475987110530308No Hit
GGAGGACCGGTCTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCT176880.1565299068316083No Hit
CATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTC165990.14689280435876675No Hit
GCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCG163850.14499901195363538No Hit
GGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTC151140.1337513009867101No Hit
CCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCG146470.12961858578485794No Hit
GGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCA145030.12834425818514333No Hit
CATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGGCGGGTGTC142930.1264858637688929No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG139040.12304340935021948No Hit
GCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTTCCT135700.12008767727865925No Hit
GATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGC121780.10776917714808493No Hit
GGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAG120920.10700812038714426No Hit
GACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTG117010.10354796697403035No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG115070.10183116451330375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC137800.052.019892
GGGCGAT139500.051.9086231
CGATCTG210650.033.9520954
TGCGACA212300.032.75868613
GCTGCGA217050.032.3756211
CTGCGAC216400.032.0261312
GGCTGCG220950.031.89783510
GCGACAT218250.031.81812114
CGACATC220000.031.17214815
GACATCT248850.027.82154716
ATAGTCG8200.026.62282870
ACATCTG263300.026.32128117
GCGATCT298450.024.456133
CTAACCG3100.023.40370211
CGGATCC36150.023.2719670
ATCTGTC307600.022.67664719
TATTGGG55650.022.19764970
CCCATTG319900.021.51410328
CATCTGT334050.020.83965518
ACGTCGA2352.0008883E-1120.5916435
Produced by FastQC (version 0.11.5)