FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9212241
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT4957.747129E-69.060532
TAGATCG4704.1496E-58.80867668
CGCGATC4855.9993887E-58.5360123
GGCGCGA6101.3539575E-68.4858391
CGCGCCC12050.08.016533
ACCGCGC15450.07.81759071
CCGCGCC17000.07.71172
GCGCCCG13951.8189894E-127.17190554
AGATCGG9908.585084E-86.969827769
CGCCCGG18850.06.9547745
GATCGGA9452.8232535E-76.936638469
CCCCCGC7157.364409E-46.27437731
GCGATCT11306.07895E-65.80077364
CGATCTT7850.00226519985.71326645
TGCGCCC9254.530357E-45.5945483
CTGCGCC13604.346013E-65.32716132
ACTGCGC12653.9958577E-55.1831821
Produced by FastQC (version 0.11.5)