FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7241607
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC10250.011.4700291
CCGCGCC12150.011.0751242
CGCGCCC8450.010.6127253
CGCGATC4159.5625255E-69.9734043
ACCGTGC12050.08.89578151
GAGTGCG4957.6399985E-58.36125241
CCGTGCC12650.07.90984062
CGTGCCC10203.110472E-107.77746253
CCGGTTT4000.0069510697.761037323
GCGCCCG9851.2532837E-97.7029094
CGCCCGG14800.07.45686965
ACCACGC20150.07.3790911
GTGCCCG8301.9333002E-67.0638124
CGGATCA9857.869676E-87.00298344
GGCGGAT11453.5270205E-96.92815642
GTCTCGT5100.0078694356.76296430
CGCGCCT7703.6735866E-56.71909573
AGTGCGG6300.00122506536.569555842
GCGGATC10753.8541657E-76.416686543
GCGCCCA9703.204179E-66.39983944
Produced by FastQC (version 0.11.5)