FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8539803
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATC3454.9130904E-913.9997553
CGCGCCC12850.013.9608443
ACCGCGC16500.013.8025871
ACCGCAC12800.013.2095061
CCGCGCC18150.012.3551562
GCGCGAT3701.8431638E-712.1214082
CGCACCC9200.011.6247963
GCGCCCG14100.011.4995974
CCGCACC12700.011.13762
CGCCCGG18300.010.9340425
TTAAGCG2550.001531397910.82378340
ACCGTGC15300.010.6000261
ACCACGC22950.09.7730731
GGCGCGA5151.2600703E-69.3803981
ACGCCCG6051.2384771E-79.1236474
CGAGGCG5008.803232E-68.96952735
GCGCCCA10350.08.6663624
GCGGATC9405.4569682E-118.44183543
ACTGCGC11800.08.1879751
CCGTGCC16900.08.1655372
Produced by FastQC (version 0.11.5)