FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7283451
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC10650.012.0119921
CCGCGCC12000.010.9271812
CGCGCCC10200.09.4685133
ACCGTGC11350.08.2249041
GGCCCGA3800.0043919038.1680836
CGCCCGG14150.08.0430675
GCGCCCG10751.4551915E-118.0203954
CCGTGCC11507.2759576E-127.8015582
CGAGGCG5552.8769908E-47.458080335
GATCGGA7654.2253105E-67.227007469
GTGCCCG8301.9335912E-67.0637464
TGCGATC5500.00213473316.8984873
ACGTAAT5050.0071230856.83304742
GCGATTC7754.0198443E-56.67572644
ACTGCGC9853.9507813E-66.3182851
GCGATCT8803.4403234E-56.27049064
CGTGCCC9451.4577183E-56.20498853
CCCGGCC33250.06.01565177
ACTTTAC30600.05.762511
ACCCGGC8650.00118018715.58178576
Produced by FastQC (version 0.11.5)