FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7230486
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC14550.015.4160961
CCGCGCC15400.014.7852992
CGCGATC3052.3366374E-713.5733893
CGCGCCC10950.013.54756453
GCGCGAT3204.1897692E-712.9371372
CGCCCGG16100.012.213865
GCGCCCG13300.011.9319134
GACGTAT2550.001528563710.8261461
ATCGGGA2250.007464532310.7330323
GGTGCGA6201.4915713E-1010.5751361
TGCGATC5552.910383E-910.5672723
TTAACGG2954.347641E-410.5250554
GCGACAG8400.010.26714218
CGAGGCG6151.4642865E-910.09699835
CGCACCC8203.6379788E-129.6765583
GCGATCT9100.09.477694
GGGCGAC9100.09.47736316
ACCGCAC11350.09.4251851
GTGCGAT6604.94947E-99.4088272
CCGGCCC9700.09.2469078
Produced by FastQC (version 0.11.5)