FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8834250
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATC4304.1836756E-1113.6391783
ACCGCGC15800.012.0121031
CCGCGCC18100.010.6737542
GCGCGAT4552.3181383E-710.6151072
GCGACAG9050.010.29219918
CGCGCCC13250.010.154453
GGGCGAC10450.09.9037116
ACCGCAC12100.09.4111361
GCGATCT11000.08.7815394
CGGATCA9457.2759576E-128.76217144
GGCGCGA5553.4722434E-68.7045941
GGCGACA13100.08.69031717
CGACAGA11150.08.66320519
GCGCCCG14750.08.6539624
ACCGTGC17450.08.3055291
GCGGATC10601.0913936E-118.13717943
CCGCACC13150.08.1328672
CGCCCGG18850.08.0527945
CTAACGA4300.00148358148.0230463
CGTGCCC12650.07.9088843
Produced by FastQC (version 0.11.5)