FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7792955
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGGT1651.8347309E-618.81430829
CGGTCAC2501.0550139E-917.93584632
TGCGGCG1959.01493E-615.91949327
ACCGCGC10750.011.9021481
CCGCGCC11750.010.8654992
CGCGCCC9150.010.5552033
ACCGCAC9800.09.1744361
ATGCGGC4201.185401E-49.03362226
GCGGTCA3500.00208463948.86937531
GGCGGTC4007.260936E-48.62305830
TAACGAT3700.00339164638.40548669
CGCCCGG14000.08.3758795
ACGCCCG5806.3306634E-68.324914
GATCGGA8554.7459253E-87.679085769
ACCGTGC11405.0931703E-117.5834581
CCGCACC11152.0081643E-97.11768532
CCGGCTA9251.9578147E-77.08375458
CGCACCC6956.9888636E-56.94820933
AAATGCG6502.3223253E-46.898358324
AATGCGG5600.0025812726.77517325
Produced by FastQC (version 0.11.5)