FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6201301
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT2900.020.2237432
ATCCGCG900.00573704719.16675451
CGCGATC3103.6379788E-1217.8061053
GGCGCGA3800.016.3446871
ACCGCGC10800.011.1823221
CGCGCCC9050.09.9114093
CCGCGCC12200.09.8973472
GCGCCCG9507.2759576E-128.7155494
GCGATCT6808.008283E-88.6247634
CGCCCGG12750.08.3880045
ACTGCGC8352.1077576E-67.02506161
GCGACAG6350.00133594136.519418
ACCGTGC12301.5405021E-86.4522381
GCCGAGG16855.456968E-126.34731333
ACCGCAC8702.8886272E-56.345831
AGATCGG6050.0057751926.2730169
ACCCGGC9401.3389832E-56.23918966
GCGCCCA8054.697337E-45.99983454
CACCCGG10601.3451498E-55.8583295
ACCACGC17751.546141E-105.8319071
Produced by FastQC (version 0.11.5)