FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6559568
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATC2901.2629789E-714.2752183
CGCGCCC8750.012.2223043
GCGCGAT3408.7351873E-712.175922
GCGCCCG9950.011.7884114
TGCGATC5905.456968E-1211.6943873
CGCCCGG13300.011.6724055
ACCGCGC10900.011.3984121
GTGCGAT6553.6379788E-1211.0605692
CGGATCA6251.4551915E-1111.04025944
CCGCGCC11250.010.7328482
GGTGCGA5856.1118044E-1010.6190331
GCGATCT8350.010.3288744
ACCGCAC9750.010.2650651
CGAGGCG4957.362887E-79.75728435
CGCACTC3956.0530838E-59.60716153
GCGGATC7657.0940587E-109.01988843
GGCGGAT7657.0940587E-109.01968242
AGCGATT8506.002665E-118.92968843
GCGATTC8152.4920155E-108.88977844
CCGCACT3905.632367E-48.8457552
Produced by FastQC (version 0.11.5)