FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5958863
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC9300.018.55321
CCGCGCC9600.016.890472
CGCGCCC7400.015.8512073
GCGCGAT2907.546987E-915.4653762
GGCGCGA3503.783498E-1014.7895521
ACCGCAC8600.013.6430731
CGCGATC2952.376839E-612.8642883
CGAGGCG3408.732695E-712.17615535
GCGCCCG8750.011.8283644
CGCCCGG11750.011.7444755
CCGCACC8600.011.2324552
CGCACCC5653.9035513E-910.3804373
GATCGAG4401.7252678E-610.19360362
GACGTAG2800.00332997419.85754951
ACGATCT4906.407299E-79.8569714
CCGGACC3157.949764E-49.85688735
ACCGTGC11950.09.8184461
GGCGGGT6151.5670594E-89.53649838
AGATCGA5503.0496813E-79.409162561
CTCACGC17750.09.1351283
Produced by FastQC (version 0.11.5)