FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6402110
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCCC7600.010.8925233
AGTTCGC2250.007473649510.73123722
ACCGCGC9700.010.6941691
CCGCGCC10550.010.1394212
TGCGATC4057.9703364E-59.3684673
ACTGCGC8709.094947E-129.1412611
CGGTTAC3050.00674925339.04797923
GCGATCT5857.474064E-78.8436664
AACCCGT3650.003031988.51030755
TGCGCCC6101.3627341E-68.4818843
GCGCCCG10305.456968E-128.3714324
ACCGCAC7554.7242793E-88.2437161
CTGCGCC8801.1241355E-98.2345632
ACTTCGA4250.00129221578.1359561
CCGTGCC10102.4920155E-107.857972
CGCCCGG13200.07.8387045
GCGCCCA7604.786016E-77.71493434
AGATCGG7251.8945648E-67.62451769
ACCGTGC10504.323738E-97.2448751
CCGAGTA8604.2867578E-77.229403566
Produced by FastQC (version 0.11.5)