FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6131948
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC8850.013.2836841
CGCGCCC7400.011.6527183
CCGCGCC10450.011.22668652
ACATCGC2550.00150350210.8475761
AGCGTCG2250.007392646710.74698665
GCGCCCG8350.09.9131544
CGCCCGG11700.09.1381725
ACCGCAC7352.2990698E-67.52688931
CTGTCGC7552.7973205E-56.851717512
CGCGCCT5650.00283357346.71526533
AGATCGG7056.134111E-46.36979565
CCGGCCA10401.4873967E-66.30055768
ACTGCGC8259.226995E-56.28666351
ACCGTGC9004.59824E-56.14695931
CCGCACC8004.3256558E-46.03847172
CGCCTGG13307.70051E-85.96430355
ACCTTAC12559.4988536E-75.78573371
CCGTCTC15757.07405E-85.4770722
ACCACGC13453.4051773E-65.39858441
CCCGGCC25300.05.31621847
Produced by FastQC (version 0.11.5)