FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8522163
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCCC11750.017.322573
ACCGCGC15500.015.8369411
CCGCGCC16600.014.9631372
CGCGATC5500.014.426583
GCGCGAT5800.014.2751772
ACGATCG1450.005221604414.274590512
ACCGCAC11200.012.3477141
GCGATCT12700.011.9521114
GCGCCCG14800.011.8878734
ACCGTGC16150.011.7742911
GGCGCGA7550.011.4482131
TGCGATC7100.011.1755193
GCGCAAC2250.00746582410.7328182
GCGCCCA11900.010.4364124
CCGCACC12000.010.06201652
CGCCCGG19600.010.0322695
ACTGCGC16300.09.9690751
CGGCCGA2800.00333381899.8562649
CCGTGCC18350.09.7760982
CGGATCA13550.09.67577744
Produced by FastQC (version 0.11.5)