FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8800222
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATC3850.016.1262113
GCGCGAT4000.015.5217432
ACCGCGC15100.015.3060421
GCGCGTC2454.9757255E-614.0786792
CGCGCCC12350.013.9644433
CCGCGCC17000.012.7826132
CGCCCGG18250.011.7177565
CGCGTCT2409.245216E-411.4973913
GGCGCGA5552.91584E-910.5662531
CCGCTCG3055.9211924E-410.17813421
CTAACGA4452.001234E-610.0763663
GGCGCGT3106.870417E-410.0148811
GCGCCCG14350.09.855024
ACGCCCG7606.366463E-119.5308414
CGAGGCG8102.1827873E-119.36776635
CACGCCC21200.09.273843
CGGATCA9300.09.27145944
GGCGGAT12100.09.12127642
GCGGATC10800.08.62245643
CCACGCC28850.08.48868752
Produced by FastQC (version 0.11.5)