FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7377600
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCGA1702.4378423E-618.2631879
CCGCACG1503.5295068E-416.0993182
ACCGCGC12650.013.9392051
GCGCGAT3001.9105937E-713.7994152
CGTCTCG1550.00811860913.35418252
ACCGCAC10750.012.8650041
CGCGATC3651.5445221E-712.287153
CGCGCCC10050.012.0144163
CCGCGCC13600.011.9222882
GCCGCAC3256.848495E-611.7022041
GGCGCGA3853.0015508E-711.6745721
CCGCACC11000.011.290432
GCGCCCG11350.010.9422664
CGCCCGG15250.010.1794825
GAGTTCG11950.09.81574962
CGCACGC2900.0044533079.5168383
GATCGAG6201.7887942E-89.45953362
AGTTCGA13800.08.99986263
ACTGCGC12750.08.9487441
ACTTCGC3905.5099104E-48.8653051
Produced by FastQC (version 0.11.5)