FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8853878
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC13750.012.7943451
CGCGCCC10550.011.1154113
GCGCCTA2500.001301310411.0370114
CCGCGCC15450.010.9387862
CTTTACG5654.1029314E-57.93590452
ACGAGTC4350.00166692487.9287986
AGCGTCG4050.007656317.677730669
GCGCCCG11901.0768417E-96.95614
ACCGCAC10654.5261913E-86.80174451
TACTGCG5100.0078717076.762798314
CGTGTAG5200.0093449736.643588565
ACCGTGC13001.0404619E-96.6335691
TTTACGA5200.0094926936.6327823
CGTGCCC10502.5180998E-76.56961253
CGCGCCT8056.808439E-56.42679453
ACTTTAC37250.06.38959551
CGCCCGG15602.401066E-106.1905625
CGCACCC7853.4104093E-46.1511663
GTGCCCG9459.6744356E-55.83968834
CCGGCCT15153.07773E-85.69117458
Produced by FastQC (version 0.11.5)