FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6881041
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCCC9100.015.5437013
GCGCGAT2101.8148823E-514.7854722
CGCGATC2304.2772277E-513.499783
ACCGCGC11400.013.0377681
CCGCGCC12400.012.2417352
GCGCCCG9300.011.4998124
CGCCCGG12150.010.7893985
GGCGCGA3004.987189E-410.3695741
ACCGTGC10750.09.003041
CGCACCC6655.5837518E-88.8194063
ACCGCAC9102.5465852E-118.7362711
GCCGCAC3200.0098375988.6413121
CCGCACC9751.2732926E-118.4921692
CCGTGCC11850.08.44292
GTGCCCG7051.4325633E-78.3190134
GCACGAT7656.133996E-88.1175152
ACCACGC17900.08.1102811
GGGCGAC7403.1170748E-77.92514316
GCGATCT8758.811185E-97.8855864
ACTGCGC10102.419256E-107.87129351
Produced by FastQC (version 0.11.5)