FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5300235
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG1155.651964E-826.9977144
CGCCGTA1101.1549491E-625.0887846
TCGCCGT1356.836013E-620.44270745
GCCGTAT2152.2677232E-514.44063347
GGTCGCC2355.235065E-513.21226743
CGTCGTG2752.2710042E-411.29005612
CCGGTAC2200.006309044510.9786231
GCGTCGT2550.001532736510.82250811
AGCGTCG2850.00385991419.683022510
GTCGTGT3905.63203E-48.845736513
CGTGTAG4100.0086584857.572670515
TCTCGGT4650.00319695987.41970536
CTCGGTG4850.0048174927.11414137
TCGGTGG5500.00212762536.90058638
ATCGGAA8300.00438986165.402645669
CGGAAGA8500.00580692855.27557468
Produced by FastQC (version 0.11.5)