FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4964798
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1551.0062995E-620.03131546
CGTATGC2651.1167298E-513.01825644
ATGCCGT2406.378115E-512.93689247
TCGTATG2551.1242776E-412.17602143
TCTCGTA2551.12470036E-412.175529541
CTCGTAT2752.270137E-411.29049242
TGCCGTC2903.7127276E-410.70639248
CGTCTTC5000.0064862226.89939851
AGATCGG13652.6045804E-55.055114768
GATCGGA13702.7741651E-55.036665469