FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4964798
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG1208.5396096E-825.87201344
CGCCGTA1251.2674536E-724.83763146
TCGCCGT1301.8516585E-723.8823445
GCCGTAT2457.740114E-512.67226147
GGTCGCC2853.163449E-410.89336943
TCTCGGT4552.3223583E-710.61341136
TGGTCGC3602.5291226E-49.5821342
TCGGTGG4552.8206073E-48.33910838
CGTATCA3800.0043808098.17027449
GTGGTCG4250.0013228188.11662841
CCGTATC4300.00148431148.02249148
TGCGGCC4100.0086667877.57183929
CGTCGTG5800.00372253616.542677412
GTCGTGT6450.0015939916.41818713
CGGTGGT5950.0048653436.37709439
CGTGTAG6050.005781256.27231915
ACCGCGC8953.97985E-56.2080931
GATCGGA14158.950239E-65.119498769
AGATCGG14351.1538381E-55.04809668
Produced by FastQC (version 0.11.5)