FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4883048
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2300.025.4990846
TCGTATG2600.021.23000343
ATGCCGT3300.018.81750347
TGCCGTC3400.018.26404848
CGTATGC3251.2369128E-1015.92250344
TCGTAGT1657.279495E-414.63583653
CTCGTAT3657.1304385E-1014.17757142
ACACGTC3751.0713848E-913.79865513
CCGACCG1800.001405041413.41632130
GCCGTCT4457.4578566E-1113.1790349
TCTCGTA4002.8103386E-912.93623841
CGTCTTC4804.179492E-810.78064151
CCGTCTT5654.5069646E-79.15877450
ATCTCGT6453.4067853E-89.09213640
ACGTCTG7353.1164745E-88.44824215
ATCGGAA6604.6341302E-78.3685622
TCGGAAG6302.1380656E-68.2191243
GATCGGA6352.3509783E-68.1646971
CGGAAGA7201.9892286E-78.1504644
GCACACG7101.4144334E-67.773889511
Produced by FastQC (version 0.11.5)