FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4991493
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGTC6050.018.24387230
ATCGTCT7150.017.36676431
CCCATCG7150.015.43712328
CTTGACG1657.289832E-414.63310530
TCGTCTG7700.013.88651932
CGATTCT11150.013.3948331
CCATCGT8950.013.10322829
GGAGCGT10900.012.9771658
AGCGTCT11600.012.78953410
GCGTCTC11450.012.35479911
CGCCCAT8300.011.63584726
GAACCGC2450.001098525611.26279827
CGCGAGC2200.006319529410.97614920
CGGCCGC12300.010.93779522
CCGAGTA10800.010.8598922
GCAATCG2250.007465884610.73266460
GCGAGCG2600.001802034610.61429821
GGCCGCC15300.010.59674223
AGGAGCG19650.010.5351727
GCGGGAT2300.00878738810.49945144
Produced by FastQC (version 0.11.5)