FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3644537
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGAC400.002426622234.98085822
ACCGCGC5650.021.7322241
CGCGCCC5350.019.664583
CCGCGCC6600.019.1287982
CGGCGGT1000.00972563417.49018929
GCGCCCG5850.016.7849244
CGCCCGG6700.014.6558955
ACCGTGC5700.012.9249541
TAAGCGG2050.003362199511.94484838
GCCCGGC11050.011.7427156
CCCGGCC13250.011.3810337
CGCGCCT2500.001132318611.221923
CCGTGCC6855.456968E-1210.7512232
GCACCCG3308.894192E-510.6268174
ACCGCAC4056.62127E-59.528451
ACTGCGC4502.0407839E-59.3552061
CACCCGG4956.290291E-69.2101625
ACGCCCA4653.0183523E-59.0499354
ACCCGGC4753.8756032E-58.8596536
CCGGCCA7101.2351484E-78.3956428
Produced by FastQC (version 0.11.5)