FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3496259
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC3850.021.8646451
ACCGTGC5250.019.0881821
CCGCGCC5000.016.8094162
ACTGCGC3408.4946805E-915.3266881
CGCGCCC4257.530616E-1014.125563
CCGTGCC6850.013.4381862
GTGCCCG3503.0559368E-612.5784764
GCGCCCG4503.400528E-711.5620334
GCGCCCA4453.7261052E-610.7925634
TGCGCCC3454.6812597E-410.4406323
CGTGCCC5453.5951416E-710.280993
CTGCGCC5101.7065595E-610.2017942
GCCCGGC7655.991751E-98.6259086
CGCCCGG4855.5233144E-58.5960965
CGTGCCT4650.0070682217.7462753
CCCGGCC9453.972673E-97.7179197
CGCCCAG6905.777989E-57.04921445
CCCTAAC9400.0032366185.54369541
ACTGCAC26251.8189894E-115.3446911
Produced by FastQC (version 0.11.5)