FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5238470
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC10550.013.0983621
CCGCGCC12150.012.2088632
CGCGCCC8900.011.240643
CGCGATC2500.001299118311.03908353
TTACGAA2250.007466586310.73254418
AGATCGG12500.09.66279669
GCGCCCA7851.1568773E-98.789084
CTTCACG4603.170042E-48.2493152
TAGATCG4250.0013174368.11999668
ACCGCAC8606.2227627E-98.0341691
ACTGCGC10608.185452E-117.82194571
GCGCCCG10001.6934791E-97.58937034
CGGATCA6958.70598E-67.44570944
TGCGCCC7009.63791E-67.3922443
CTGCGCC10554.984031E-97.1937162
CGCACCC6703.35719E-46.69347433
GCGGATC7301.325862E-46.61614243
GCGACAG6600.00206527286.272266418
ACCGTGC9501.54282E-56.18208171
CCGCACC9501.5775076E-56.1731722
Produced by FastQC (version 0.11.5)