FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6374935
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATC2002.8880095E-818.97424536
TATGCCG2053.8315193E-818.51189446
TGCGGCG1800.001404939513.416553543
TGCCGTC3601.2900091E-712.45813248
CGTATGC3651.5438673E-712.28747144
CCGCGAA2000.003099218712.07575169
CGCATCT3451.0425374E-611.99895837
TCTCGTA4359.782525E-911.89551841
ATGCCGT4652.6184352E-811.12885147
GGCGCTT4753.581772E-810.8945646
CTGGCGC4754.1869134E-710.16801633
GAGCGTC12900.010.1618289
GGAGCGT13450.010.002778
CGGCGCT3451.6459107E-49.99983745
TCGTATG4201.09926805E-59.857059543
CGATCCT10550.09.8278441
TCGTCTG9100.09.47771932
AGCGTCT14350.09.3754210
AACCGGG5553.4795266E-79.32402730
CATCGTC8951.8189894E-129.25110130
Produced by FastQC (version 0.11.5)