FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5442146
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG5301.6874267E-47.81052469
CGTCGTG5502.5891984E-47.526504569
GATCGGA16050.07.522597369
TTAACGT4150.0096618337.48020235
GCGTCGT5350.0015918967.092734369
GCGACAG7301.7402133E-57.08812351
CATCGTC6007.028932E-46.89872630
AGATCGG16900.06.73587568
CACGGAG7301.3272061E-46.615641663
AGCGTCG6350.00133701266.518900468
GAAGGCC23150.06.448861
CCCATCG7552.057873E-46.39616928
AGCGTCT11401.6385457E-76.355432510
GAGGTAA27050.06.2891841
CGGCCAC6050.0057859246.27185742
GCGTCTC11602.2708628E-76.24625811
TCGTCTG6650.00225112146.224356732
GGGCGAC7309.500144E-46.143208549
CGACAGA8551.556108E-46.051847552
GGCCGCC16702.0372681E-105.990516723
Produced by FastQC (version 0.11.5)