FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005333961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005333961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6414699
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA1409.358935E-619.71316146
GTCGCCG1653.848156E-516.72618744
TCGCCGT1907.0328715E-616.34117145
TACGGAT2300.00878866410.49931948
CGTATCA3501.9038314E-49.856503549
GCCGTAT2850.00385800689.68365847
GGTCGCC3150.0087894618.76113143
CCGTATC3600.00268749688.6244448
GCGTCGT5806.3127936E-68.326591567
CGTCGTG6352.1185928E-57.60527268
GTGGTCG5350.00159229977.09261141
AGCGTCG6855.769779E-57.050252466
TCGGTGG5950.00486668946.376991738
GTCGTGT8054.702974E-45.99928469
CGGTGGT7400.0073950885.59362139
AGATCGG16704.4625267E-85.37061669
GATCGGA16401.7012462E-75.25851869
CGGAAGA17003.6670826E-75.07292369
Produced by FastQC (version 0.11.5)