FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005334021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005334021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5391390
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA1103.67163E-828.22480246
GTCGCCG1403.7849532E-722.17621844
TCGCCGT1601.3648714E-619.4045545
GCCGTAT2609.180962E-613.26806947
GGTCGCC3501.9046733E-49.85600643
TGGTCGC3652.905275E-49.45078942
CGTATCA3350.00139741069.26758749
TTTACGA3050.00674337439.04889213
GCGTCGT3500.00207749228.87262611
TAGATCG3956.3993526E-48.73308969
CGTCGTG4007.246084E-48.62472412
CCGTATC4000.00694594677.76160448
TCTCGGT4800.00436827967.186071436
AGATCGG8754.4068056E-66.702021669
GATCGGA9150.00238926995.27803169
Produced by FastQC (version 0.11.5)